MilliporeSigma Microbial Monitoring Tools
| Step | Equipment / Material | Description | Product |
|---|---|---|---|
| 1 | Vacuum Source or Pump | Provides the vacuum required to pull a sample through the membrane | EX-Stream Pump |
| 2 | Manifold or Filtering Flask | Provides the support for the membrane and funnel as well as an exit for the liquid | EZ-Fit Manifold |
| 3 | Funnel or Filter Holder | Holds the sample during filtration | EZ-Microfil Funnel Devices |
| 4 | Membrane | Retains the target microbes contained in the liquid sample | EZ-Pak Membranes |
| 5 | Petri-Dish and Pad | Dishes used to combine membrane and media | Petri-Pad Petri-Dish |
| 6 | Growth Media | Substance to provide growing nutrients in either liquid or agar form | WL Sterile Ampoule |
| 7 | Incubator and Colony Counter | Provides controlled conditions to grow microbial colonies which can be counted |
EZ-Stream™ Pump
The new MilliporeSigma EZ-Stream vacuum filtration pump is specifically designed for microbiological analysis. The compact design is ideal for both the work bench and the laminar flow hood. The vacuum is provided by a maintenance-free diaphragm. Check valves allow liquids to run directly through the pump. There is no need to use any waste containers normally associated with traditional air pumps or an in-house vacuum, eliminating the need for repeated emptying of heavy waste containers. The EZ-Stream has also been optimized for maximum noise reduction during operation. Watch EZ-Stream Pump Video
EZ-Fit™ Manifold
The first truly plug-and-play manifold compatible with multiple formats of filtration tulips. The MilliporeSigma EZ-Fit manifold is designed to be sanitary and low profile, operates with quick connect fittings for easy disassembly or adaptation to meet a variety of needs. No tools are needed for disassembly and all areas are easily cleaned. Works with all formats of reusable or sterile disposable funnels or membrane holders. Watch EZ-Fit Manifold Video
EZ-Pak® Curve
The MilliporeSigma EZ-Pak Curve is the next generation of EZ-Pak sterile membrane dispenser from MilliporeSigma. It features a motion activated sensor for automatic and hands-free dispensing of a sterile membrane for micro-plating or filterability work. The design allows for fast and easy loading of EZ-Pak membrane bands. Compatible with all current EZ-Pak membranes. Watch EZ-Pak Curve Video
EZ-Pak Membranes
Smaller volume labs may prefer the S-Pak, individually wrapped and pre-sterilized membrane filter disks. Microfil® funnels can be purchased bundled with both EZ-Pak and S-Pak filters for easy ordering. MilliporeSigma’s Microfil manifold is specifically designed for ease of use with funnels.
Liquid Media and Petri-Pad® Petri Dishes
Ready-to-use 2 ml plastic ampoules of sterile liquid media save time and effort when you only have a few samples to culture. No need to prepare media, simply uncap the ampoule and squeeze the sterile media into a Petri-Pad Petri dish’s absorbent pad. Petri-Pad sterile 47 mm petri dishes with preloaded pads come fully assembled and ready to use. Each pad conveniently absorbs 2 ml of liquid media to instantly prepare it to receive an EZ-Pak or S-Pak membrane filter for culturing. View MilliporeSigma Liquid Ampoule Culture Media Wine Application Guide
Granulated (Dehydrated) Media
Granulated media offers several key advantages over traditional dehydrated media including: each granule contains the exact ingredient proportions and no settling or separation of media ingredients that can lead to inconsistent tests. It also has faster dissolution, easier to weigh, reduced dust and inhaling of particles and no sticking to glassware that can cause media waste and inconsistent results. MilliporeSigma specifies the highest performance criteria available on the market in its media quality certificates. Additional media formulations and versions available.
Brewing Microbial Monitoring and ATP Testing
- Microbial Monitoring in the Brewery
- Common Testing Procedures
- Tools Used to Test for Microbes
- Common Testing Points and Troubleshooting
- ATP Testing and Theory
- Presentation Notes: Microbial Monitoring and ATP Testing Slides
